Conjugatable water-soluble Pt(II) and Pd(II) porphyrin complexes: novel nano- and molecular probes for optical oxygen tension measurement in tissue engineering.
Photochem Photobiol Sci
; 13(7): 1039-51, 2014 Jul.
Article
em En
| MEDLINE
| ID: mdl-24818569
ABSTRACT
Measurement of oxygen tension in compressed collagen sheets was performed using matrix-embedded optical oxygen sensors based on platinum(II) and palladium(II) porphyrins supported on polyacrylamide nanoparticles. Bespoke, fully water-soluble, mono-functionalised Pt(II) and Pd(II) porphyrin complexes designed for conjugation under mild conditions were obtained using microwave-assisted metallation. The new sensors display a linear response (1/τ vs. O2) to varying oxygen tension over a biologically relevant range (7.0 × 10(-4) to 2.7 × 10(-1) mM) in aqueous solutions; a behaviour that is maintained following conjugation to polyacrylamide nanoparticles, and following embedding of the nanosensors in compressed collagen sheets, paving the way to innovative approaches for real-time resolution of oxygen gradients throughout 3D matrices useful for tissue regeneration.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Oxigênio
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Paládio
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Platina
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Porfirinas
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Espectrometria de Fluorescência
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Sondas Moleculares
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Complexos de Coordenação
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article