ALIX is recruited temporarily into HIV-1 budding sites at the end of gag assembly.
PLoS One
; 9(5): e96950, 2014.
Article
em En
| MEDLINE
| ID: mdl-24834918
ABSTRACT
Polymerization of Gag on the inner leaflet of the plasma membrane drives the assembly of Human Immunodeficiency Virus 1 (HIV-1). Gag recruits components of the endosomal sorting complexes required for transport (ESCRT) to facilitate membrane fission and virion release. ESCRT assembly is initiated by recruitment of ALIX and TSG101/ESCRT-I, which bind directly to the viral Gag protein and then recruit the downstream ESCRT-III and VPS4 factors to complete the budding process. In contrast to previous models, we show that ALIX is recruited transiently at the end of Gag assembly, and that most ALIX molecules are recycled into the cytosol as the virus buds, although a subset remains within the virion. Our experiments imply that ALIX is recruited to the neck of the assembling virion and is mostly recycled after virion release.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
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Proteínas de Ligação ao Cálcio
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HIV-1
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Proteínas de Ciclo Celular
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Montagem de Vírus
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Proteínas de Ligação a DNA
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Produtos do Gene gag do Vírus da Imunodeficiência Humana
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Complexos Endossomais de Distribuição Requeridos para Transporte
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article