Immunocytochemical staining of endogenous nuclear proteins with the HIS-1 anti-poly-histidine monoclonal antibody: a potential source of error in His-tagged protein detection.

Chilumuri, Amrutha; Markiv, Anatoliy; Milton, Nathaniel G N

Chilumuri, Amrutha; Markiv, Anatoliy; Milton, Nathaniel G N.

Afiliação

Chilumuri A; Department of Human and Health Sciences, Faculty of Science and Technology, University of Westminster, 115 New Cavendish Street, London W1W 6UW, UK.
Markiv A; Department of Biomedical Sciences, Faculty of Science and Technology, University of Westminster, 115 New Cavendish Street, London W1W 6UW, UK.
Milton NG; Department of Human and Health Sciences, Faculty of Science and Technology, University of Westminster, 115 New Cavendish Street, London W1W 6UW, UK; Department of Biomedical Sciences, Faculty of Science and Technology, University of Westminster, 115 New Cavendish Street, London W1W 6UW, UK. Electronic address: n.milton@westminster.ac.uk.

Acta Histochem ; 116(6): 1022-8, 2014 Jul.

Article em En | MEDLINE | ID: mdl-24835186

Assuntos

Anticorpos Monoclonais Murinos/química; Artefatos; Catalase/metabolismo; Linhagem Celular Tumoral; Técnica Indireta de Fluorescência para Anticorpo; Histidina/imunologia; Humanos; Proteínas Nucleares/metabolismo; Oligopeptídeos/imunologia; Proteínas Recombinantes de Fusão/metabolismo

Palavras-chave

Anti-polyhistidine; Catalase overexpression; His-tagged proteins; Immunocytochemistry; SH-SY5Y neuroblastoma; Transfection

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Anticorpos Monoclonais Murinos Idioma: En Ano de publicação: 2014 Tipo de documento: Article

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Anticorpos Monoclonais Murinos Idioma: En Ano de publicação: 2014 Tipo de documento: Article