[Chemical reactions in double-helical nucleic acids. VIII. Assembly of hybrid RNA-DNA-duplexes using water soluble condensing agents]. / Khimicheskie reaktsii v dvukhspiral'nykh nukleinovykh kislotakh. VIII. Sborka gibridnykh RNK-DNK dupleksov s ispol'zovaniem vodorasvorimykh kondensiruiushchikh agentov.

ABSTRACT

Condensation of oligonucleotides containing ribonucleotide segments (from mononucleotide to full sequence) on DNA-template was studied, with cyanogen bromide and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide as condensing agents. Efficiency of the chemical ligation of RNA oligomers was much lower than that of DNA analogues. The yield of chemical ligation products was found to depend on the position of RNA segment in the hybrid duplexes and on the position of the phosphate group in the nick. The oligoribonucleotide's length does not affect the yield of condensation products. A nick in 5S RNA loop was repaired by means of the chemical ligation method.