A recently introduced Dichelobacter nodosus strain caused an outbreak of footrot in Norway.
Acta Vet Scand
; 56: 29, 2014 May 13.
Article
em En
| MEDLINE
| ID: mdl-24886510
ABSTRACT
BACKGROUND:
In 2008, an outbreak of ovine footrot occurred in Norway. Dichelobacter nodosus isolates collected between 2008 and 2011 have been characterised. Isolates defined as virulent by the gelatin gel test (GG-test) were only found in sheep in Rogaland County, where the severe cases of footrot were registered. The majority (96%) of the virulent isolates belonged to serogroup A. It is suspected that they represent a newly introduced strain, and the aim of the present study was to investigate whether they are genetically similar. Sixty-one virulent isolates from sheep and 116 benign isolates from sheep, cattle and goats were included. Four GG-test virulent isolates from Danish sheep were also included. All isolates were genotyped by pulsed-field gel electrophoresis (PFGE) and by PCR for pgr variant determination.RESULTS:
The Norwegian virulent isolates were assigned to 8 pulsotypes (PTs), while the benign isolates were assigned to 66 PTs. Thirty-seven (68.5%) of the 54, virulent, serogroup A isolates belonged to the same PT, and included isolates from 2008 through 2011. Isolates belonging to this PT were defined as the outbreak strain. The remaining virulent serogroup A isolates belonged to 4 PTs differing by ≤3 bands from the outbreak strain. Two virulent, Danish, serogroup A isolates differed by 2 bands from the Norwegian outbreak strain. All but 3 (95%) of the virulent isolates had the pgrA variant while 85% of the benign isolates had the pgrB variant.CONCLUSION:
This study provides evidence that the footrot outbreak in Norway in 2008 most likely was caused by new introduction and local spread of one virulent D. nodosus strain.
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Base de dados:
MEDLINE
Assunto principal:
Doenças dos Ovinos
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Doenças dos Bovinos
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Doenças das Cabras
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Infecções por Bactérias Gram-Negativas
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Dichelobacter nodosus
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Pododermatite Necrótica dos Ovinos
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article