A simple, robust and rapid approach to detect carbapenemases in Gram-negative isolates by MALDI-TOF mass spectrometry: validation with triple quadripole tandem mass spectrometry, microarray and PCR.
Clin Microbiol Infect
; 20(12): O1106-12, 2014 Dec.
Article
em En
| MEDLINE
| ID: mdl-24930405
ABSTRACT
Carbapenemases should be accurately and rapidly detected, given their possible epidemiological spread and their impact on treatment options. Here, we developed a simple, easy and rapid matrix-assisted laser desorption ionization-time of flight (MALDI-TOF)-based assay to detect carbapenemases and compared this innovative test with four other diagnostic approaches on 47 clinical isolates. Tandem mass spectrometry (MS-MS) was also used to determine accurately the amount of antibiotic present in the supernatant after 1 h of incubation and both MALDI-TOF and MS-MS approaches exhibited a 100% sensitivity and a 100% specificity. By comparison, molecular genetic techniques (Check-MDR Carba PCR and Check-MDR CT103 microarray) showed a 90.5% sensitivity and a 100% specificity, as two strains of Aeromonas were not detected because their chromosomal carbapenemase is not targeted by probes used in both kits. Altogether, this innovative MALDI-TOF-based approach that uses a stable 10-µg disk of ertapenem was highly efficient in detecting carbapenemase, with a sensitivity higher than that of PCR and microarray.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
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Beta-Lactamases
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Técnicas Bacteriológicas
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Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
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Bactérias Gram-Negativas
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article