Nicotine effect on bone remodeling during orthodontic tooth movement: histological study in rats.
Dental Press J Orthod
; 19(2): 96-107, 2014.
Article
em En
| MEDLINE
| ID: mdl-24945520
ABSTRACT
INTRODUCTION:
Nicotine is harmful to angiogenesis, osteogenesis and synthesis of collagen.OBJECTIVE:
The aim of this study was to investigate the effect of nicotine on bone remodeling during orthodontic movement in rats.METHODS:
Eighty male Wistar rats were randomly divided into three groups Group C (control), group CM (with orthodontic movement) and group NM (nicotine with orthodontic movement) groups. The animals comprising groups C and CM received 0.9% saline solution while group NM received nicotine solution (2 mg/kg). A nickel-titanium closed-coil spring was used to induce tooth movement. The animals were euthanized and tissue specimens were histologically processed. Blood vessels, Howship's lacunae and osteoclast-like cells present in the tension and compression areas of periodontal ligaments were quantified. The extent of bone formation was evaluated under polarized light, to determine the percentage of immature/mature collagen.RESULTS:
It was observed lower blood vessel densities in the NM group in comparison to the CM group, three (p < 0.001) and seven (p < 0.05) days after force application. Osteoclast-like cells and Howship's lacunae in the NM group presented lower levels of expression, in comparison to the CM group, with significant differences on day 7 (p < 0.05 for both variables) and day 14 (p < 0.05 for osteoclast-like cells and p < 0.01 for Howship's lacunae). The percentage of immature collagen was increased in the NM group in comparison to the CM group, with a statistically significant difference on day 3 (p < 0.05), day 7 (p < 0.001), day 14 (p < 0.001) and day 21 (p < 0.001).CONCLUSIONS:
Nicotine affects bone remodeling during orthodontic movement, reducing angiogenesis, osteoclast-like cells and Howship's lacunae, thereby delaying the collagen maturation process in new bone matrix.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Ligamento Periodontal
/
Técnicas de Movimentação Dentária
/
Remodelação Óssea
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Processo Alveolar
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Nicotina
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article