Profiling lysine ubiquitination by selective enrichment of ubiquitin remnant-containing peptides.
Methods Mol Biol
; 1174: 57-71, 2014.
Article
em En
| MEDLINE
| ID: mdl-24947374
ABSTRACT
Protein ubiquitination plays critical roles in many biological processes. However, functional studies of protein ubiquitination in eukaryotic cells are limited by the ability to identify protein ubiquitination sites. Unbiased high-throughput screening methods are necessary to discover novel ubiquitination sites that play important roles in cellular regulation. Here, we describe an immunopurification approach that enriches ubiquitin remnant-containing peptides to facilitate downstream mass spectrometry (MS) identification of lysine ubiquitination sites. This approach can be utilized to identify ubiquitination sites from proteins in a complex mixture.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Ubiquitina
/
Proteômica
/
Ubiquitinação
/
Lisina
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article