Comparison of cardioprotective effects of labeled and unlabeled oleanoic acids with new BOPIM dye on primary neonatal rat cardiomyocytes following hypoxia/reoxygenation injury.
Pharmacol Rep
; 66(4): 677-85, 2014 Aug.
Article
em En
| MEDLINE
| ID: mdl-24948071
ABSTRACT
BACKGROUND:
It is well known that fluorescent labeling has recently become a major research tool in molecular and cellular biology for demonstrating therapeutic mechanisms and metabolic pathways. However, few studies have reported the use of fluorescent labeling of natural products.METHODS:
We recently explored the boron 2-(2'-pyridyl) imidazole (BOPIM) derivative analogs, which are highly fluorescent, non-aggregated, and nontoxic. In the present study, the natural product oleanolic acid (OA) was functionalized and labeled with BOPIM, thus yielding a highly fluorescent probe, the comparison of cardioprotective effects of labeled and unlabeled OAs with BOPIM on primary neonatal rat cardiomyocytes with hypoxia/reoxygenation (H/R) injury were investigated.RESULTS:
Pretreatment with OA and BOPIM-OA significantly prevented the H/R induced cell death in primary neonatal rat cardiomyocytes. However, BOPIM exhibited no improvements on the H/R injury cardiomyocytes, and which were similar to those of the H/R group. The results of comparison of cardioprotective effects between labeled and unlabeled OAs with BOPIM showed that introducing the BOPIM chromophore did not make a difference with H/R injury cardiomyocytes.CONCLUSION:
BOPIM chromophore is a suitable probe for investigating the pharmacological mechanisms of natural products.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Ácido Oleanólico
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Oxigênio
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Compostos de Boro
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Cardiotônicos
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Miócitos Cardíacos
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Corantes Fluorescentes
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article