Optimization of fed-batch enzymatic hydrolysis from alkali-pretreated sugarcane bagasse for high-concentration sugar production.
Bioresour Technol
; 167: 41-5, 2014 Sep.
Article
em En
| MEDLINE
| ID: mdl-24968110
ABSTRACT
Fed-batch enzymatic hydrolysis process from alkali-pretreated sugarcane bagasse was investigated to increase solids loading, produce high-concentration fermentable sugar and finally to reduce the cost of the production process. The optimal initial solids loading, feeding time and quantities were examined. The hydrolysis system was initiated with 12% (w/v) solids loading in flasks, where 7% fresh solids were fed consecutively at 6h, 12h, 24h to get a final solids loading of 33%. All the requested cellulase loading (10 FPU/g substrate) was added completely at the beginning of hydrolysis reaction. After 120 h of hydrolysis, the maximal concentrations of cellobiose, glucose and xylose obtained were 9.376 g/L, 129.50 g/L, 56.03 g/L, respectively. The final total glucan conversion rate attained to 60% from this fed-batch process.
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Base de dados:
MEDLINE
Assunto principal:
Carboidratos
/
Celulose
/
Álcalis
/
Técnicas de Cultura Celular por Lotes
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article