α-lipoic acid reduces fatty acid esterification and lipogenesis in adipocytes from overweight/obese subjects.
Obesity (Silver Spring)
; 22(10): 2210-5, 2014 Oct.
Article
em En
| MEDLINE
| ID: mdl-25045030
ABSTRACT
OBJECTIVE:
α-Lipoic acid (α-LA) is a natural occurring antioxidant with beneficial effects on obesity. The aim of this study was to investigate the putative effects of α-LA on triglyceride accumulation and lipogenesis in subcutaneous adipocytes from overweight/obese subjects and to determine the potential mechanisms involved.METHODS:
Fully differentiated human subcutaneous adipocytes were treated with α-LA (100 and 250 µM) during 24 h for studying triglyceride content, de novo lipogenesis, and levels of key lipogenic enzymes. The involvement of AMP-activated protein kinase (AMPK) activation was also evaluated.RESULTS:
α-LA down-regulated triglyceride content by inhibiting fatty acid esterification and de novo lipogenesis. These effects were mediated by reduction in fatty acid synthase (FAS), stearoyl-coenzyme A desaturase 1, and diacylglycerol O-acyltransferase 1 protein levels. Interestingly, α-LA increased AMPK and acetyl CoA carboxylase phosphorylation, while the presence of the AMPK inhibitor Compound C reversed the inhibition observed on FAS protein levels.CONCLUSIONS:
α-LA down-regulates key lipogenic enzymes, inhibiting lipogenesis and reducing triglyceride accumulation through the activation of AMPK signaling pathway in human subcutaneous adipocytes from overweight/obese subjects.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Ácido Tióctico
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Adipócitos
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Sobrepeso
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Metabolismo dos Lipídeos
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Lipogênese
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Ácidos Graxos
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Obesidade
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article