The neutral self-assembling peptide hydrogel SPG-178 as a topical hemostatic agent.
PLoS One
; 9(7): e102778, 2014.
Article
em En
| MEDLINE
| ID: mdl-25047639
ABSTRACT
Conventional self-assembling peptide hydrogels are effective as topical hemostatic agents. However, there is a possibility to harm living tissues due to their low pH. The aim of the present study was to demonstrate the efficacy of SPG-178, a neutral self-assembling peptide hydrogel, as a topical hemostatic agent. First, we measured the bleeding duration of incisions made on rat livers after application of SPG-178 (1.0% w/v), SPG-178 (1.5% w/v), RADA16 (1.0% w/v), and saline (nâ=â12/group). Second, we observed the bleeding surfaces by transmission electron microscopy immediately after hemostasis. Third, we measured the elastic and viscous responses (G' and Gâ³, respectively) of the hydrogels using a rheometer. Our results showed that bleeding duration was significantly shorter in the SPG-178 group than in the RADA16 group and that there were no significant differences in transmission electron microscopy findings between the groups. The greater the G' value of a hydrogel, the shorter was the bleeding duration. We concluded that SPG-178 is more effective and has several advantages it is non-biological, transparent, nonadherent, and neutral and can be sterilized by autoclaving.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Hemostáticos
/
Hidrogéis
/
Hemorragia
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article