Biosynthetic pathway of the phytohormone auxin in insects and screening of its inhibitors.
Insect Biochem Mol Biol
; 53: 66-72, 2014 Oct.
Article
em En
| MEDLINE
| ID: mdl-25111299
ABSTRACT
Insect galls are abnormal plant tissues induced by galling insects. The galls are used for food and habitation, and the phytohormone auxin, produced by the insects, may be involved in their formation. We found that the silkworm, a non-galling insect, also produces an active form of auxin, indole-3-acetic acid (IAA), by de novo synthesis from tryptophan (Trp). A detailed metabolic analysis of IAA using IAA synthetic enzymes from silkworms indicated an IAA biosynthetic pathway composed of a three-step conversion Trp â indole-3-acetaldoxime â indole-3-acetaldehyde (IAAld) â IAA, of which the first step is limiting IAA production. This pathway was shown to also operate in gall-inducing sawfly. Screening of a chemical library identified two compounds that showed strong inhibitory activities on the conversion step IAAld â IAA. The inhibitors can be efficiently used to demonstrate the importance of insect-synthesized auxin in gall formation in the future.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Reguladores de Crescimento de Plantas
/
Bombyx
/
Inibidores Enzimáticos
/
Vias Biossintéticas
/
Himenópteros
/
Ácidos Indolacéticos
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article