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Tracheal tissue engineering in rats.
Jungebluth, Philipp; Haag, Johannes C; Sjöqvist, Sebastian; Gustafsson, Ylva; Beltrán Rodríguez, Antonio; Del Gaudio, Costantino; Bianco, Alessandra; Dehnisch, Ivar; Uhlén, Per; Baiguera, Silvia; Lemon, Greg; Lim, Mei Ling; Macchiarini, Paolo.
Afiliação
  • Jungebluth P; Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose and Throat, Stockholm, Sweden.
  • Haag JC; Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose and Throat, Stockholm, Sweden.
  • Sjöqvist S; Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose and Throat, Stockholm, Sweden.
  • Gustafsson Y; Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose and Throat, Stockholm, Sweden.
  • Beltrán Rodríguez A; Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose and Throat, Stockholm, Sweden.
  • Del Gaudio C; Department of Enterprise Engineering, University of Rome, Intrauniversity Consortium for Material Science and Technology (INSTM), Research Unit 'Tor Vergata', Rome, Italy.
  • Bianco A; Department of Enterprise Engineering, University of Rome, Intrauniversity Consortium for Material Science and Technology (INSTM), Research Unit 'Tor Vergata', Rome, Italy.
  • Dehnisch I; Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
  • Uhlén P; Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
  • Baiguera S; Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose and Throat, Stockholm, Sweden.
  • Lemon G; Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose and Throat, Stockholm, Sweden.
  • Lim ML; Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose and Throat, Stockholm, Sweden.
  • Macchiarini P; Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose and Throat, Stockholm, Sweden.
Nat Protoc ; 9(9): 2164-79, 2014 Sep.
Article em En | MEDLINE | ID: mdl-25122525
ABSTRACT
Tissue-engineered tracheal transplants have been successfully performed clinically. However, before becoming a routine clinical procedure, further preclinical studies are necessary to determine the underlying mechanisms of in situ tissue regeneration. Here we describe a protocol using a tissue engineering strategy and orthotopic transplantation of either natural decellularized donor tracheae or artificial electrospun nanofiber scaffolds into a rat model. The protocol includes details regarding how to assess the scaffolds' biomechanical properties and cell viability before implantation. It is a reliable and reproducible model that can be used to investigate the crucial aspects and pathways of in situ tracheal tissue restoration and regeneration. The model can be established in <6 months, and it may also provide a means to investigate cell-surface interactions, cell differentiation and stem cell fate.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Traqueia / Engenharia Tecidual / Regeneração Tecidual Guiada / Alicerces Teciduais Idioma: En Ano de publicação: 2014 Tipo de documento: Article
Texto completo
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XML
PubMed Links
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Traqueia / Engenharia Tecidual / Regeneração Tecidual Guiada / Alicerces Teciduais Idioma: En Ano de publicação: 2014 Tipo de documento: Article