[Inhibitory effect of genistein on the proliferation of Raji cells and its related mechanism].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
; 22(4): 971-5, 2014 Aug.
Article
em Zh
| MEDLINE
| ID: mdl-25130812
ABSTRACT
This study was aimed to investigate the anti-proliferative effect of genistein (Gen) on BCL-6 positive Raji cells and its related mechanism. Trypan blue staining and MTT method were used to analyze the anti-proliferative effect of Gen on Raji cells. Cell apoptosis, protein expression and the interaction of BCL-6 and NCoR were detected by PI/AV dual staining, Western blot and Co-IP method, respectively. The results showed that Gen had time- and dose-dependent inhibitory effect on Raji cell proliferation and induced apoptosis. Different dose of Gen had no significant effect on the expression of BCL-6 and NCoR, but could inhibit the binding of BCL-6 and NCoR. It is concluded that Gen shows inhibitory effect on BCL-6 positive lymphoma cells, which can be as a adjuvant therapy for combined rituximab with chemotherapy.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Linfócitos
/
Genisteína
/
Proliferação de Células
Idioma:
Zh
Ano de publicação:
2014
Tipo de documento:
Article