Direct identification of bacteria from positive BacT/ALERT blood culture bottles using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry.
Diagn Microbiol Infect Dis
; 80(3): 193-6, 2014 Nov.
Article
em En
| MEDLINE
| ID: mdl-25139844
ABSTRACT
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry is a fast and robust method for the identification of bacteria. In this study, we evaluate the performance of a laboratory-developed lysis method (LDT) for the rapid identification of bacteria from positive BacT/ALERT blood culture bottles. Of the 168 positive bottles tested, 159 were monomicrobial, the majority of which were Gram-positive organisms (61.0% versus 39.0%). Using a cut-off score of ≥1.7, 80.4% of the organisms were correctly identified to the species level, and the identification rate of Gram-negative organisms (90.3%) was found to be significantly greater than that of Gram-positive organisms (78.4%). The simplicity and cost-effectiveness of the LDT enable it to be fully integrated into the routine workflow of the clinical microbiology laboratory, allowing for rapid identification of Gram-positive and Gram-negative bacteria within an hour of blood culture positivity.
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Base de dados:
MEDLINE
Assunto principal:
Bactérias
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Sangue
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Técnicas Bacteriológicas
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Bacteriemia
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Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article