Chromatographic and electrophoretic strategies for the chiral separation and quantification of d- and l-threo methylphenidate in biological matrices.
Biomed Chromatogr
; 28(11): 1554-64, 2014 Nov.
Article
em En
| MEDLINE
| ID: mdl-25196779
ABSTRACT
Commercially available methylphenidate (MPH) exists as a racemic mixture composed of the d- and l-threo enantiomers. Various pharmacokinetic studies of MPH have shown a greater pharmacological potency of the d-threo enantiomer. Furthermore, it was deduced that the stereoselective cleavage of MPH to produce ritalinic acid (RA) by human carboxylesterase results in a higher oral bioavailability of the d-threo enantiomer. As a requirement for pharmaceutical regulation authorities, efforts have been made to determine the differential biological distribution of d- and l-threo MPH and RA enantiomers. In support of these efforts, numerous analytical procedures have been developed for the chiral separation and quantification of MPH enantiomers in a variety of biological matrices. The available methodologies accomplish the enantioseparation and quantification of MPH using gas chromatography, liquid chromatography or capillary electrophoretic techniques coupled with a variety of detectors. The current review discusses the technical procedures involved, and the sensitivity and selectivity of these assays.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Cromatografia Líquida
/
Eletroforese Capilar
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Cromatografia Gasosa-Espectrometria de Massas
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Metilfenidato
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article