RGDechi-hCit: αvß3 selective pro-apoptotic peptide as potential carrier for drug delivery into melanoma metastatic cells.
PLoS One
; 9(9): e106441, 2014.
Article
em En
| MEDLINE
| ID: mdl-25248000
ABSTRACT
αvß3 integrin is an important tumor marker widely expressed on the surface of cancer cells. Recently, we reported some biological features of RGDechi-hCit, an αvß3 selective peptide antagonist. In the present work, we mainly investigated the pro-apoptotic activity of the molecule and its ability to penetrate the membrane of WM266 cells, human malignant melanoma cells expressing high levels of αvß3 integrin. For the first time we demonstrated the pro-apoptotic effect and the ability of RGDechi-hCit to enter into cell overexpressing αvß3 integrin mainly by clathrin- and caveolin-mediated endocytosis. Furthermore, we deepened and confirmed the selectivity, anti-adhesion, and anti-proliferative features of the peptide. Altogether these experiments give insight into the biological behavior of RGDechi-hCit and have important implications for the employment of the peptide as a new selective carrier to deliver drugs into the cell and as a therapeutic and diagnostic tool for metastatic melanoma. Moreover, since the peptide shows a pro-apoptotic effect, a great perspective could be the development of a new class of selective systems containing RGDechi-hCit and pro-apoptotic molecules or other therapeutic agents to attain a synergic action.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Inibidores da Angiogênese
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Integrina alfaVbeta3
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Melanoma
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article