Novel and sensitive qPCR assays for the detection and identification of aspergillosis causing species.
Acta Microbiol Immunol Hung
; 61(3): 273-84, 2014 Sep.
Article
em En
| MEDLINE
| ID: mdl-25261942
ABSTRACT
Despite concerted efforts, diagnosis of aspergillosis is still a great challenge to clinical microbiology laboratories. Along with the requirement for high sensitivity and specificity, species-specific identification is important. We developed rapid, sensitive and species-specific qPCR assays using the TaqMan technology for the detection and identification of Aspergillus fumigatus and Aspergillus terreus. The assays were designed to target orthologs of the Streptomyces factor C gene that are only found in a few species of filamentous fungi. Fungi acquired this gene through horizontal gene transfer and divergence of the gene allows identification of species. The assays have potential as a molecular diagnosis tool for the early detection of fungal infection caused by Aspergillus fumigatus and Aspergillus terreus, which merits future diagnostic studies. The assays were sensitive enough to detect a few genomic equivalents in blood samples.
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Base de dados:
MEDLINE
Assunto principal:
Aspergilose
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Aspergillus
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Reação em Cadeia da Polimerase
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article