Imaging callose at plasmodesmata using aniline blue: quantitative confocal microscopy.
Methods Mol Biol
; 1217: 105-19, 2015.
Article
em En
| MEDLINE
| ID: mdl-25287199
ABSTRACT
Callose (ß-1,3-glucan) is both structural and functional component of plasmodesmata (Pd). The turnover of callose at Pd controls the cell-to-cell diffusion rate of molecules through Pd. An accurate assessment of changes in levels of Pd-associated callose has become a first-choice experimental approach in the research of intercellular communication in plants.Here we describe a detailed and easy-to-perform procedure for imaging and quantification of Pd-associated callose using fixed plant tissue stained with aniline blue. We also introduce an automated image analysis protocol for non-biased quantification of callose levels at Pd from fluorescence images using ImageJ. Two experimental examples of Pd-callose quantification using the automated method are provided as well.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Nicotiana
/
Parede Celular
/
Arabidopsis
/
Folhas de Planta
/
Plasmodesmos
/
Glucanos
/
Compostos de Anilina
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article