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Analysis of the expression patterns, subcellular localisations and interaction partners of Drosophila proteins using a pigP protein trap library.
Lowe, Nick; Rees, Johanna S; Roote, John; Ryder, Ed; Armean, Irina M; Johnson, Glynnis; Drummond, Emma; Spriggs, Helen; Drummond, Jenny; Magbanua, Jose P; Naylor, Huw; Sanson, Bénédicte; Bastock, Rebecca; Huelsmann, Sven; Trovisco, Vitor; Landgraf, Matthias; Knowles-Barley, Seymour; Armstrong, J Douglas; White-Cooper, Helen; Hansen, Celia; Phillips, Roger G; Lilley, Kathryn S; Russell, Steven; St Johnston, Daniel.
Afiliação
  • Lowe N; The Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK.
  • Rees JS; The Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK The Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK.
  • Roote J; The Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK.
  • Ryder E; The Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK.
  • Armean IM; The Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK.
  • Johnson G; The Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK.
  • Drummond E; The Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK.
  • Spriggs H; The Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK.
  • Drummond J; The Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK.
  • Magbanua JP; The Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK.
  • Naylor H; The Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK.
  • Sanson B; The Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK.
  • Bastock R; The Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK.
  • Huelsmann S; The Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK.
  • Trovisco V; The Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK.
  • Landgraf M; The Department of Zoology, University of Cambridge, Downing Street, Cambridge CB2 3EJ, UK.
  • Knowles-Barley S; Institute for Adaptive and Neural Computation, University of Edinburgh, 10 Crichton Street, Edinburgh EH8 9AB, UK.
  • Armstrong JD; Institute for Adaptive and Neural Computation, University of Edinburgh, 10 Crichton Street, Edinburgh EH8 9AB, UK.
  • White-Cooper H; Cardiff School of Biosciences, The Sir Martin Evans Building, Museum Avenue, Cardiff CF10 3AX, UK.
  • Hansen C; Department of Genetics, University of Leicester, Adrian Building, University Road, Leicester LE1 7RH, UK.
  • Phillips RG; Centre for Advanced Microscopy, University of Sussex, School of Life Sciences, John Maynard Smith Building, Falmer, Brighton and Hove BN1 9QG, UK.
  • Lilley KS; The Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK.
  • Russell S; The Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK.
  • St Johnston D; The Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK d.stjohnston@gurdon.cam.ac.uk.
Development ; 141(20): 3994-4005, 2014 Oct.
Article em En | MEDLINE | ID: mdl-25294943
Although we now have a wealth of information on the transcription patterns of all the genes in the Drosophila genome, much less is known about the properties of the encoded proteins. To provide information on the expression patterns and subcellular localisations of many proteins in parallel, we have performed a large-scale protein trap screen using a hybrid piggyBac vector carrying an artificial exon encoding yellow fluorescent protein (YFP) and protein affinity tags. From screening 41 million embryos, we recovered 616 verified independent YFP-positive lines representing protein traps in 374 genes, two-thirds of which had not been tagged in previous P element protein trap screens. Over 20 different research groups then characterized the expression patterns of the tagged proteins in a variety of tissues and at several developmental stages. In parallel, we purified many of the tagged proteins from embryos using the affinity tags and identified co-purifying proteins by mass spectrometry. The fly stocks are publicly available through the Kyoto Drosophila Genetics Resource Center. All our data are available via an open access database (Flannotator), which provides comprehensive information on the expression patterns, subcellular localisations and in vivo interaction partners of the trapped proteins. Our resource substantially increases the number of available protein traps in Drosophila and identifies new markers for cellular organelles and structures.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Regulação da Expressão Gênica no Desenvolvimento / Perfilação da Expressão Gênica / Proteínas de Drosophila / Drosophila melanogaster / Proteínas de Membrana Idioma: En Ano de publicação: 2014 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Regulação da Expressão Gênica no Desenvolvimento / Perfilação da Expressão Gênica / Proteínas de Drosophila / Drosophila melanogaster / Proteínas de Membrana Idioma: En Ano de publicação: 2014 Tipo de documento: Article