Selenate reductase activity in Escherichia coli requires Isc iron-sulfur cluster biosynthesis genes.
FEMS Microbiol Lett
; 361(2): 138-43, 2014 Dec.
Article
em En
| MEDLINE
| ID: mdl-25307727
ABSTRACT
The selenate reductase in Escherichia coli is a multi-subunit enzyme predicted to bind Fe-S clusters. In this study, we examined the iron-sulfur cluster biosynthesis genes that are required for selenate reductase activity. Mutants devoid of either the iscU or hscB gene in the Isc iron-sulfur cluster biosynthesis pathway lost the ability to reduce selenate. Genetic complementation by the wild-type sequences restored selenate reductase activity. The results indicate the Isc biosynthetic system plays a key role in selenate reductase Fe-S cofactor assembly and is essential for enzyme activity.
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Base de dados:
MEDLINE
Assunto principal:
Oxirredutases
/
Enxofre
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Escherichia coli
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Ferro
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article