Rapid differentiation of in vitro cellular responses to toxic chemicals by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
Environ Toxicol Chem
; 34(1): 161-6, 2015 Jan.
Article
em En
| MEDLINE
| ID: mdl-25319019
ABSTRACT
Changes in protein expression as a cellular response to chemical exposure have been well established. Current methods for monitoring cellular responses usually require the use of specific reagents and/or labor-intensive procedures. The present study demonstrates the concept of using mass spectral pattern to distinguish different cellular responses. The concept is based on the ability to acquire a unique mass spectral pattern directly from a specific cell culture by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The results demonstrate that distinguishable and reproducible spectral patterns can be obtained from different cellular responses.
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Base de dados:
MEDLINE
Assunto principal:
Aflatoxina B1
/
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
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Proteoma
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Peróxido de Hidrogênio
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article