Evaluation of a stool antigen test using a mAb for native catalase for diagnosis of Helicobacter pylori infection in children and adults.
J Med Microbiol
; 63(Pt 12): 1621-1625, 2014 Dec.
Article
em En
| MEDLINE
| ID: mdl-25332372
ABSTRACT
Non-invasive diagnosis of Helicobacter pylori infection is important not only for screening of infection but also for epidemiological studies. Stool antigen tests are non-invasive and are convenient to identify H. pylori infection, particularly in children. We evaluated the stool antigen test, which uses a mAb for native catalase of H. pylori developed in Japan. A total of 151 stool samples were collected from participants (52 children and 99 adults) of the Sasayama Cohort Study and stored between -30 and -80 °C. The stool antigen test used was Testmate pylori antigen (TPAg), and was performed according to the manufacturer's instructions. Furthermore, we conducted a quantitative real-time PCR test and compared the PCR results with those of the TPAg test. When compared with the results in real-time PCR, the sensitivity of TPAg was 89.5â% overall, 82.7â% for children and 92.4â% for adults, and the specificity was 100â%. The accuracy was 93.4â% overall, 90.4â% for children and 94.9â% for adults, and there was no significant difference in the accuracy of TPAg between children and adults. Five of 28 children (18â%) and five of 38 adults (13â%) were PCR positive with negative TPAg results. Four of five children with positive PCR and negative TPAg results were given a (13)C-urea breath test and all four children tested negative. No significant correlation was observed between the TPAg results and DNA numbers of H. pylori in faeces among children or adults. A stool antigen test (TPAg) using a mAb for native catalase is useful for diagnosis of H. pylori in children and adults. Additionally, this test has particularly high specificity.
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Base de dados:
MEDLINE
Assunto principal:
Catalase
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Helicobacter pylori
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Infecções por Helicobacter
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Testes Diagnósticos de Rotina
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Fezes
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Anticorpos Antibacterianos
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Anticorpos Monoclonais
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article