RNA tertiary structure analysis by 2'-hydroxyl molecular interference.
Biochemistry
; 53(43): 6825-33, 2014 Nov 04.
Article
em En
| MEDLINE
| ID: mdl-25341083
ABSTRACT
We introduce a melded chemical and computational approach for probing and modeling higher-order intramolecular tertiary interactions in RNA. 2'-Hydroxyl molecular interference (HMX) identifies nucleotides in highly packed regions of an RNA by exploiting the ability of bulky adducts at the 2'-hydroxyl position to disrupt overall RNA structure. HMX was found to be exceptionally selective for quantitative detection of higher-order and tertiary interactions. When incorporated as experimental constraints in discrete molecular dynamics simulations, HMX information yielded accurate three-dimensional models, emphasizing the power of molecular interference to guide RNA tertiary structure analysis and fold refinement. In the case of a large, multidomain RNA, the Tetrahymena group I intron, HMX identified multiple distinct sets of tertiary structure interaction groups in a single, concise experiment.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Tetrahymena
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Bacillus subtilis
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RNA Bacteriano
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RNA de Protozoário
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Escherichia coli
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Conformação de Ácido Nucleico
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article