Robust HPV-18 production in organotypic cultures of primary human keratinocytes.
Methods Mol Biol
; 1249: 93-109, 2015.
Article
em En
| MEDLINE
| ID: mdl-25348300
ABSTRACT
The productive program of the human papillomaviruses takes place in terminally differentiating squamous epithelia. In this chapter, we provide the protocols for robust production of HPV-18 in organotypic cultures of early passages of primary human keratinocytes. A critical step is the generation of genomic HPV plasmids in vivo by using Cre-loxP-mediated excisional recombination from a vector plasmid. We discuss the rationale for this approach. This system produces high yields of infectious virus and facilitates genetic analyses of HPV protein functions and their regulation in the context of recapitulated host tissue environment.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Queratinócitos
/
Técnicas de Cultura de Células
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Papillomavirus Humano 18
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article