The use of optical trap and microbeam to investigate the mechanical and transport characteristics of tunneling nanotubes in tumor spheroids.
J Biophotonics
; 8(9): 694-704, 2015 Sep.
Article
em En
| MEDLINE
| ID: mdl-25355694
ABSTRACT
The use of optical trap and microbeam for investigating mechanical and transport properties of inter cellular tunneling nanotubes (TnTs) in tumor spheroids has been demonstrated. TnTs in tumor spheroids have been visualized by manipulating TnT connected cells using optical tweezers. Functionality of the TnTs for transferring cytoplasmic vesicles and injected dye molecules by optoporation method has been studied. Further, the TnTs could be longitudinally stretched by manipulating the connected cells and their elastic response was studied. Manipulation of cells at the surface of tumor spheroid using optical tweezers and injection of fluorescent dye into a trapped cell using optoporation technique.
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Base de dados:
MEDLINE
Assunto principal:
Esferoides Celulares
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Pinças Ópticas
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Fenômenos Mecânicos
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article