Tetracycline-based system for controlled inducible expression of group III metabotropic glutamate receptors.
J Biomol Screen
; 20(3): 350-8, 2015 Mar.
Article
em En
| MEDLINE
| ID: mdl-25394730
ABSTRACT
A stable and inducible expression of metabotropic glutamate receptor type 4, 7, and 8 was obtained in T-REx 293 cells using the tetracycline system. Tetracycline administration to the cell medium resulted in rapid induction and time-dependent expression of mGlu receptors, which also correlates with its functionality in a cAMP accumulation assay. The pharmacological properties of recombinant mGlu receptors were verified using orthosteric and allosteric ligands. Data suggest that the Tet-on inducible system is suitable for functional mGlu receptors' expression and characterization by means of the cAMP accumulation assay. It makes this system a precise, reproducible, and large-scale screening method, as well as a reasonable tool to study signaling properties of mGlu receptors.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Tetraciclina
/
Regulação da Expressão Gênica
/
Receptores de Glutamato Metabotrópico
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article