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NanoFlares for the detection, isolation, and culture of live tumor cells from human blood.
Halo, Tiffany L; McMahon, Kaylin M; Angeloni, Nicholas L; Xu, Yilin; Wang, Wei; Chinen, Alyssa B; Malin, Dmitry; Strekalova, Elena; Cryns, Vincent L; Cheng, Chonghui; Mirkin, Chad A; Thaxton, C Shad.
Afiliação
  • Halo TL; Department of Chemistry, International Institute for Nanotechnology, Northwestern University, Evanston, IL 60208-3113;
  • McMahon KM; Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Chicago, IL 60611; Simpson Querrey Institute for BioNanotechnology in Medicine, Northwestern University, Chicago, IL, 60611; Walter S. and Lucienne Driskill Graduate Training Program in Life Sciences, Northwestern University, Chic
  • Angeloni NL; Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Chicago, IL 60611; Simpson Querrey Institute for BioNanotechnology in Medicine, Northwestern University, Chicago, IL, 60611; Department of Urology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611;
  • Xu Y; Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Chicago, IL 60611; Department of Medicine, Division of Hematology/Oncology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611.
  • Wang W; Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Chicago, IL 60611; Department of Medicine, Division of Hematology/Oncology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611.
  • Chinen AB; Department of Chemistry, International Institute for Nanotechnology, Northwestern University, Evanston, IL 60208-3113;
  • Malin D; Division of Endocrinology, Diabetes and Metabolism, Department of Medicine, University of Wisconsin Carbone Cancer Center, University of Wisconsin School of Medicine and Public Health, Madison, WI, 53705; and.
  • Strekalova E; Division of Endocrinology, Diabetes and Metabolism, Department of Medicine, University of Wisconsin Carbone Cancer Center, University of Wisconsin School of Medicine and Public Health, Madison, WI, 53705; and.
  • Cryns VL; Division of Endocrinology, Diabetes and Metabolism, Department of Medicine, University of Wisconsin Carbone Cancer Center, University of Wisconsin School of Medicine and Public Health, Madison, WI, 53705; and.
  • Cheng C; Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Chicago, IL 60611; Department of Medicine, Division of Hematology/Oncology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611 cthaxton003@md.northwestern.edu chadnano@northwestern.edu chengc@northwestern.edu.
  • Mirkin CA; Department of Chemistry, International Institute for Nanotechnology, Northwestern University, Evanston, IL 60208-3113; Department of Materials Science and Engineering, and cthaxton003@md.northwestern.edu chadnano@northwestern.edu chengc@northwestern.edu.
  • Thaxton CS; International Institute for Nanotechnology, Northwestern University, Evanston, IL 60208-3113; Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Chicago, IL 60611; Simpson Querrey Institute for BioNanotechnology in Medicine, Northwestern University, Chicago, IL, 60611; Department
Proc Natl Acad Sci U S A ; 111(48): 17104-9, 2014 Dec 02.
Article em En | MEDLINE | ID: mdl-25404304
ABSTRACT
Metastasis portends a poor prognosis for cancer patients. Primary tumor cells disseminate through the bloodstream before the appearance of detectable metastatic lesions. The analysis of cancer cells in blood­so-called circulating tumor cells (CTCs)­may provide unprecedented opportunities for metastatic risk assessment and investigation. NanoFlares are nanoconstructs that enable live-cell detection of intracellular mRNA. NanoFlares, when coupled with flow cytometry, can be used to fluorescently detect genetic markers of CTCs in the context of whole blood. They allow one to detect as few as 100 live cancer cells per mL of blood and subsequently culture those cells. This technique can also be used to detect CTCs in a murine model of metastatic breast cancer. As such, NanoFlares provide, to our knowledge, the first genetic-based approach for detecting, isolating, and characterizing live cancer cells from blood and may provide new opportunities for cancer diagnosis, prognosis, and personalized therapy.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Carbocianinas / DNA Antissenso / Nanopartículas Metálicas / Ouro / Células Neoplásicas Circulantes Idioma: En Ano de publicação: 2014 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Carbocianinas / DNA Antissenso / Nanopartículas Metálicas / Ouro / Células Neoplásicas Circulantes Idioma: En Ano de publicação: 2014 Tipo de documento: Article