CpG island-mediated global gene regulatory modes in mouse embryonic stem cells.
Nat Commun
; 5: 5490, 2014 Nov 18.
Article
em En
| MEDLINE
| ID: mdl-25405324
ABSTRACT
Both transcriptional and epigenetic regulations are fundamental for the control of eukaryotic gene expression. Here we perform a compendium analysis of >200 large sequencing data sets to elucidate the regulatory logic of global gene expression programs in mouse embryonic stem (ES) cells. We define four major classes of DNA-binding proteins (Core, PRC, MYC and CTCF) based on their target co-occupancy, and discover reciprocal regulation between the MYC and PRC classes for the activity of nearly all genes under the control of the CpG island (CGI)-containing promoters. This CGI-dependent regulatory mode explains the functional segregation between CGI-containing and CGI-less genes during early development. By defining active enhancers based on the co-occupancy of the Core class, we further demonstrate their additive roles in CGI-containing gene expression and cell type-specific roles in CGI-less gene expression. Altogether, our analyses provide novel insights into previously unknown CGI-dependent global gene regulatory modes.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Regulação da Expressão Gênica
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Ilhas de CpG
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Metilação de DNA
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Proteínas de Ligação a DNA
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Células-Tronco Embrionárias
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article