In vivo shRNA screens in solid tumors.
Nat Protoc
; 9(12): 2880-902, 2014 Dec.
Article
em En
| MEDLINE
| ID: mdl-25411954
ABSTRACT
Loss-of-function (LOF) experiments targeting multiple genes during tumorigenesis can be implemented using pooled shRNA libraries. RNAi screens in animal models rely on the use of multiple shRNAs to simultaneously disrupt gene function, as well as to serve as barcodes for cell fate outcomes during tumorigenesis. Here we provide a protocol for performing RNAi screens in orthotopic mouse tumor models, referring to glioma and lung adenocarcinoma as specific examples. The protocol aims to provide guidelines for applying RNAi to a diverse spectrum of solid tumors and to highlight crucial considerations when designing and performing these studies. It covers shRNA library assembly and packaging into lentiviral particles, and transduction into tumor-initiating cells (TICs), followed by in vivo transplantation, tumor DNA recovery, sequencing and analysis. Depending on the target genes and tumor model, tumor suppressors and oncogenes can be identified or biological pathways can be dissected in 6-9 weeks.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Técnicas Genéticas
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Marcação de Genes
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RNA Interferente Pequeno
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Neoplasias Experimentais
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article