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A label-free and sensitive fluorescent method for the detection of uracil-DNA glycosylase activity.
Tao, Jing; Song, Panshu; Sato, Yusuke; Nishizawa, Seiichi; Teramae, Norio; Tong, Aijun; Xiang, Yu.
Afiliação
  • Tao J; Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084, China. xiang-yu@tsinghua.edu.cn.
Chem Commun (Camb) ; 51(5): 929-32, 2015 Jan 18.
Article em En | MEDLINE | ID: mdl-25435323
The activity of uracil-DNA glycosylase (UDG), an enzyme in the base excision repair, is detected at a high sensitivity by a DNA substrate containing only one uracil through a label-free fluorescent approach, which is also successfully applied for the measurement of UDG inhibitors.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Uracila-DNA Glicosidase / Ensaios Enzimáticos Idioma: En Ano de publicação: 2015 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Uracila-DNA Glicosidase / Ensaios Enzimáticos Idioma: En Ano de publicação: 2015 Tipo de documento: Article