Random insertion and gene disruption via transposon mutagenesis of Ureaplasma parvum using a mini-transposon plasmid.

Aboklaish, Ali F; Dordet-Frisoni, Emilie; Citti, Christine; Toleman, Mark A; Glass, John I; Spiller, O Brad

Aboklaish, Ali F; Dordet-Frisoni, Emilie; Citti, Christine; Toleman, Mark A; Glass, John I; Spiller, O Brad.

Afiliação

Aboklaish AF; Cardiff University, School of Medicine, Department of Child Health, 5th floor University Hospital of Wales, Cardiff CF14 4XN, UK; Sebha University, Faculty of Engineering and Technology, Medical Laboratory Sciences Department, PO Box 68, Libya.
Dordet-Frisoni E; INRA, UMR 1225, IHAP, 31076 Toulouse, France; Université de Toulouse, INP, ENVT, UMR1225, IHAP, 31076 Toulouse, France.
Citti C; INRA, UMR 1225, IHAP, 31076 Toulouse, France; Université de Toulouse, INP, ENVT, UMR1225, IHAP, 31076 Toulouse, France.
Toleman MA; Cardiff University, School of Medicine, Institute of Infection and Immunity, Cardiff CF14 4XN, UK.
Glass JI; J. Craig Venter Institute, Rockville, MD, USA.
Spiller OB; Cardiff University, School of Medicine, Department of Child Health, 5th floor University Hospital of Wales, Cardiff CF14 4XN, UK. Electronic address: SpillerB@cardiff.ac.uk.

Int J Med Microbiol ; 304(8): 1218-25, 2014 Nov.

Article em En | MEDLINE | ID: mdl-25444567

Assuntos

Elementos de DNA Transponíveis; Técnicas de Inativação de Genes; Genética Microbiana/métodos; Mutagênese Insercional/métodos; Ureaplasma/genética; Plasmídeos; Transformação Bacteriana

Palavras-chave

Gene disruption; RNA helicase; Transposon mutagenesis; Ureaplasma parvum; Ureaplasma urealyticum

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ureaplasma / Elementos de DNA Transponíveis / Mutagênese Insercional / Técnicas de Inativação de Genes / Genética Microbiana Idioma: En Ano de publicação: 2014 Tipo de documento: Article

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google