Perdeuteration: improved visualization of solvent structure in neutron macromolecular crystallography.
Acta Crystallogr D Biol Crystallogr
; 70(Pt 12): 3266-72, 2014 Dec 01.
Article
em En
| MEDLINE
| ID: mdl-25478844
ABSTRACT
The 1.8â
Å resolution neutron structure of deuterated type III antifreeze protein in which the methyl groups of leucine and valine residues are selectively protonated is presented. Comparison between this and the 1.85â
Å resolution neutron structure of perdeuterated type III antifreeze protein indicates that perdeuteration improves the visibility of solvent molecules located in close vicinity to hydrophobic residues, as cancellation effects between H atoms of the methyl groups and nearby heavy-water molecules (D2O) are avoided.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Perciformes
/
Proteínas Anticongelantes Tipo III
/
Proteínas de Peixes
/
Difração de Nêutrons
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article