Two-stage gene assembly/cloning of a member of the TspDTI subfamily of bifunctional restriction endonucleases, TthHB27I.

Krefft, Daria; Zylicz-Stachula, Agnieszka; Mulkiewicz, Ewa; Papkov, Aliaksei; Jezewska-Frackowiak, Joanna; Skowron, Piotr M

Krefft, Daria; Zylicz-Stachula, Agnieszka; Mulkiewicz, Ewa; Papkov, Aliaksei; Jezewska-Frackowiak, Joanna; Skowron, Piotr M.

Afiliação

Krefft D; Department of Molecular Biotechnology, Institute for Environmental and Human Health Protection, Division of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, Poland. Electronic address: daria.krefft@phdstud.ug.edu.pl.
Zylicz-Stachula A; Department of Molecular Biotechnology, Institute for Environmental and Human Health Protection, Division of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, Poland. Electronic address: a.zylicz-stachula@ug.edu.pl.
Mulkiewicz E; Department of Environment Analysis, Institute for Environmental and Human Health Protection, Division of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, Poland. Electronic address: ewa.mulkiewicz@ug.edu.pl.
Papkov A; Department of Molecular Biotechnology, Institute for Environmental and Human Health Protection, Division of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, Poland. Electronic address: aliaksei.papkov@ug.edu.pl.
Jezewska-Frackowiak J; Department of Molecular Biotechnology, Institute for Environmental and Human Health Protection, Division of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, Poland. Electronic address: j.jezewska-frackowiak@ug.edu.pl.
Skowron PM; Department of Molecular Biotechnology, Institute for Environmental and Human Health Protection, Division of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, Poland. Electronic address: piotr.skowron@ug.edu.pl.

J Biotechnol ; 194: 67-80, 2015 Jan 20.

Article em En | MEDLINE | ID: mdl-25486633

Assuntos

Enzimas de Restrição do DNA/metabolismo; Enzimas de Restrição do DNA/genética; Desoxirribonucleases de Sítio Específico do Tipo II/genética; Desoxirribonucleases de Sítio Específico do Tipo II/metabolismo; Escherichia coli/genética; Escherichia coli/metabolismo; Thermus/enzimologia

Palavras-chave

Methyltransferase; Restriction endonuclease; Thermus; Type IIG; Type IIS

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Enzimas de Restrição do DNA Idioma: En Ano de publicação: 2015 Tipo de documento: Article

Texto completo

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Enzimas de Restrição do DNA Idioma: En Ano de publicação: 2015 Tipo de documento: Article