Physicochemical properties of complex rhamnogalacturonan I from gelatinous cell walls of flax fibers.
Carbohydr Polym
; 117: 853-861, 2015 Mar 06.
Article
em En
| MEDLINE
| ID: mdl-25498709
ABSTRACT
The physicochemical properties of flax fiber cell wall rhamnogalacturonan I (RG-I) and its fragments, obtained after galactanase treatment (fraction G1), were characterized. RG-I retains its hydrodynamic volume after its molecular weight decreases by approximately half, as revealed by SEC. Two techniques, DLS and NMR, with different principles of diffusion experiment were used to establish the reasons for this property of RG-I. Three possible types of particles were revealed by DLS depending on the concentration of the RG-I and G1 solutions (2-2.5, 15-20, and 150-200 nm). It was determined by BPP-LED experiments that the backbone of the RG-I was 1.3-1.9-fold more mobile than the side chains. The obtained data suggest a novel type of pectin spatial organization-the formation of RG-I associates with the backbone at the periphery and the interaction between the side chains to form a core zone.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Parede Celular
/
Pectinas
/
Linho
/
Gelatina
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article