Decreased serum level of miR-146a as sign of chronic inflammation in type 2 diabetic patients.
PLoS One
; 9(12): e115209, 2014.
Article
em En
| MEDLINE
| ID: mdl-25500583
ABSTRACT
BACKGROUND:
There is increasing evidence that chronic inflammation is an important determinant in insulin resistance and in the pathogenesis of type 2 diabetes (T2D). MicroRNAs constitute a newly discovered system of cell regulation and in particular two microRNAs (miR-146a and miR-155) have been described as regulators and biomarkers of inflammation.AIM:
To determine a putative association between the levels of miR-146a and miR-155 in serum of T2D patients, clinical parameters and serological indicators of inflammation.METHODS:
We performed quantitative Real Time PCR (qPCR) of microRNAs from serum (56 Ecuadorian T2D ambulatory patients and 40 non-diabetic controls). In addition, we evaluated T2D-related serum cytokines.chemokines and growth factors using a commercially available multi-analyte cytometric bead array system. We correlated outcomes to clinical parameters, including BMI, HbA1c and lipid state.RESULTS:
The Ecuadorian non-diabetic controls appeared as overweight (BMI>25 patients 85%, controls 82.5%) and as dyslipidemic (hypercholesterolemia patients 60.7%, controls 67.5%) as the patients. The serum levels of miR-146a were significantly reduced in T2D patients as compared to these non-diabetic, but obese/dyslipidemic control group (mean patients 0.61, mean controls set at 1; pâ=â0.042), those of miR-155 were normal.The serum levels of both microRNAs correlated to each other (râ=â0.478; p<0.001) and to leptin levels. The microRNAs did not correlate to BMI, glycemia and dyslipidemia.From the tested cytokines, chemokines and growth factors, we found IL-8 and HGF significantly raised in T2D patients versus non-diabetic controls (pâ=â0.011 and 0.023 respectively).CONCLUSIONS:
This study shows decreased serum anti-inflammatory miR-146a, increased pro-inflammatory IL-8 and increased HGF (a vascular/insular repair factor) as discriminating markers of failure of glucose control occurring on the background of obesity and dyslipidemia.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
MicroRNAs
/
Diabetes Mellitus Tipo 2
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article