Usefulness of microRNA375 as a prognostic and therapeutic tool in esophageal squamous cell carcinoma.
Int J Oncol
; 46(3): 1059-66, 2015 Mar.
Article
em En
| MEDLINE
| ID: mdl-25501018
ABSTRACT
The aim of this study was to clarify the importance of microRNA375 (miR375) expression in patients with esophageal squamous cell carcinoma (ESCC) and to examine the in vivo antitumor effects of miR375 in a model of ESCC using a nonviral delivery system. We estimated the miR375 and LDHB and AEG1/MTDH mRNA expression of the ESCC tumors from 85 patients. The correlation between the miR375 expression and clinicopathological features, including the prognosis, were evaluated. The presence of high miR375 expression was associated with lymphatic vessel invasion, while a low expression of miR375 significantly correlated with a poor prognosis for the 85 ESCC patients. We also found that there was a significant inverse correlation between the expression of miR375 and that of LDHB. Before the examination of miR375 in the in vivo assay, we confirmed that atelocollagen prolonged the accumulation of miRNA by using fluorescentlylabeled miRNA and an in vivo imaging system. We injected the miR375/atelocollagen complex or a controlmiRNA/atelocollagen complex into mice bearing TE2 and T.Tn xenografts via subcutaneous (s.c.) injections. The growth of both the TE2 and T.Tn tumors in the miR375 groups was significantly suppressed compared with that in the controlmiRNA groups. In addition, The LDHB mRNA expression of TE2 xenografts was significantly downregulated after miR375 treatment. In conclusion, it might be possible for the level of miR375 expression to be a utilized as a prognostic indicator for ESCC patients. The administration of miR375 using a nonviral delivery might represent a powerful new treatment for ESCC.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias Esofágicas
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Carcinoma de Células Escamosas
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Regulação Neoplásica da Expressão Gênica
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MicroRNAs
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article