Numerical and structural chromosomal anomalies in undifferentiated pleomorphic sarcoma.
Anticancer Res
; 34(12): 7119-27, 2014 Dec.
Article
em En
| MEDLINE
| ID: mdl-25503139
ABSTRACT
BACKGROUND:
Malignant fibrous histiocytoma (MFH) or undifferentiated pleomorphic sarcoma (UPS) is the most common soft-tissue sarcoma of late adult life. Further advances in genetic characterization are warranted. The aim of this study was to search for numerical and structural chromosomal anomalies in UPS. MATERIALS ANDMETHODS:
We investigated five sarcoma-specific chromosomal translocations, five oncogene amplifications as well as the numerical karyotype of 19 UPS samples and one UPS/MFH cell line (U2197) using FISH probes on interphase nuclei.RESULTS:
Our results demonstrate that chromosomal translocations involving CHOP, SYT, EWS, FUS and FKHR genes are absent. Furthermore, amplification of ERBB2 (10.5%) and MDM2 (10.5%) was observed whereas the EGFR, C-MYC and N-MYC genes were not amplified. Interestingly, predominant aneuploidies were found in eight chromosomes.CONCLUSION:
The data demonstrate rarity of sarcoma-specific chromosomal breaks and oncogene amplifications in UPS, yet polysomic chromosomes appear more characteristically in this condition.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Translocação Genética
/
Amplificação de Genes
/
Quebra Cromossômica
/
Histiocitoma Fibroso Maligno
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article