High-throughput platform assay technology for the discovery of pre-microrna-selective small molecule probes.
Bioconjug Chem
; 26(1): 19-23, 2015 Jan 21.
Article
em En
| MEDLINE
| ID: mdl-25506628
ABSTRACT
MicroRNAs (miRNA) play critical roles in human development and disease. As such, the targeting of miRNAs is considered attractive as a novel therapeutic strategy. A major bottleneck toward this goal, however, has been the identification of small molecule probes that are specific for select RNAs and methods that will facilitate such discovery efforts. Using pre-microRNAs as proof-of-concept, herein we report a conceptually new and innovative approach for assaying RNA-small molecule interactions. Through this platform assay technology, which we term catalytic enzyme-linked click chemistry assay or cat-ELCCA, we have designed a method that can be implemented in high throughput, is virtually free of false readouts, and is general for all nucleic acids. Through cat-ELCCA, we envision the discovery of selective small molecule ligands for disease-relevant miRNAs to promote the field of RNA-targeted drug discovery and further our understanding of the role of miRNAs in cellular biology.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Sondas Moleculares
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MicroRNAs
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Descoberta de Drogas
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Ensaios de Triagem em Larga Escala
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article