Rapid and scalable assembly of firefly luciferase substrates.
Org Biomol Chem
; 13(7): 2117-21, 2015 Feb 21.
Article
em En
| MEDLINE
| ID: mdl-25525906
ABSTRACT
Bioluminescence imaging with luciferase-luciferin pairs is a popular method for visualizing biological processes in vivo. Unfortunately, most luciferins are difficult to access and remain prohibitively expensive for some imaging applications. Here we report cost-effective and efficient syntheses of d-luciferin and 6'-aminoluciferin, two widely used bioluminescent substrates. Our approach employs inexpensive anilines and Appel's salt to generate the luciferin cores in a single pot. Additionally, the syntheses are scalable and can provide multi-gram quantities of both substrates. The streamlined production and improved accessibility of luciferin reagents will bolster in vivo imaging efforts.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Luciferina de Vaga-Lumes
/
Luciferases de Vaga-Lume
/
Substâncias Luminescentes
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article