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Screening for miRNAs and their potential targets in response to TGF-ß1 based on miRNA microarray and comparative proteomics analyses in a mouse GC-1 spg germ cell line.
Rong, Zhuoxian; Li, Dan; Liu, Xiaowen; Liu, Zhiyong; Wu, Daobing; Liu, Xuanming.
Afiliação
  • Rong Z; Department of Life Science, College of Biology, Hunan University, Changsha, Hunan 410082, P.R. China.
  • Li D; Department of Life Science, College of Biology, Hunan University, Changsha, Hunan 410082, P.R. China.
  • Liu X; Department of Life Science, College of Biology, Hunan University, Changsha, Hunan 410082, P.R. China.
  • Liu Z; Department of Life Science, College of Biology, Hunan University, Changsha, Hunan 410082, P.R. China.
  • Wu D; Department of Life Science, College of Biology, Hunan University, Changsha, Hunan 410082, P.R. China.
  • Liu X; Department of Life Science, College of Biology, Hunan University, Changsha, Hunan 410082, P.R. China.
Int J Mol Med ; 35(3): 821-8, 2015 Mar.
Article em En | MEDLINE | ID: mdl-25573148
Transforming growth factor-ß1 (TGF-ß1) is a member of the TGF-ß superfamily that performs a number of cellular functions and shows differential activity at different testicular developmental stages. In the present study, we investigated the effects of exogenous TGF-ß1 on global microRNA (miRNA or miR) expression profiles by miRNA microarray analysis and the alterations in protein profiles by two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/TOF MS) in a mouse GC-1 spg germ cell line. A total of 24 differentially expressed miRNAs, including 7 upregulated and 17 downregulated miRNAs were identified. The results obtained by the RT-qPCR analysis of 10 selected differentially expressed miRNAs were in accordance with those obtained by miRNA microarray analysis. In addition, 11 differentially expressed proteins, including 3 upregulated and 8 downregulated proteins were identified through MS-based comparative proteomics analysis. Bioinformatics analysis predicted that peptidyl­prolyl isomerase A (PPIA) and nucleoside diphosphate kinase B (NDKB) are targets of miR-149 and miR-199a-3p, respectively in response to the stimulation of mouse GC-1 spg germ cells with TGF-ß1. RT-qPCR revealed that the expression levels of these miRNAs showed an opposite trend in response to stimulation with TGF-ß1. In conclusion, we identified some important miRNAs and proteins as possible targets involved in TGF-ß1 signaling. Our data suggest the existence of a TGF-ß1­miR­149-PPIA or TGF-ß1-miR-199a-3p-NDKB pathway in GC-1 spg cells. Further studies are warranted to ascertain the role of these miRNAs in spermatogenesis.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: RNA Mensageiro / Regulação da Expressão Gênica / MicroRNAs / Proteômica / Fator de Crescimento Transformador beta1 / Células Germinativas Idioma: En Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: RNA Mensageiro / Regulação da Expressão Gênica / MicroRNAs / Proteômica / Fator de Crescimento Transformador beta1 / Células Germinativas Idioma: En Ano de publicação: 2015 Tipo de documento: Article