Ferric microperoxidase-11 catalyzes peroxynitrite isomerization.
J Inorg Biochem
; 144: 56-61, 2015 Mar.
Article
em En
| MEDLINE
| ID: mdl-25578411
ABSTRACT
Microperoxidase-11 (MP11) is an undecapeptide derived from horse heart cytochrome c offering the possibility to study the reactivity of the heme group relatively unshielded by the protein. Here, the peroxynitrite isomerization to NO3(-) catalyzed by ferric MP11 (MP11-Fe(III)) is reported. Data were obtained between pH3.6 and 8.1, at 20.0°C. The value of the second-order rate constant (kon) for peroxynitrite isomerization to NO3(-) by MP11-Fe(III) decreases from (1.1±0.1)×10(5)M(-1)s(-1), at pH3.6, to (6.1±0.6)×10(3)M(-1)s(-1), at pH8.1. The pH dependence of kon (pKa=6.9) suggests that peroxynitrous acid reacts preferentially with MP11-Fe(III). The MP11-Fe(III)-catalyzed isomerization of peroxynitrite to NO3(-) has been ascribed to the reactive penta-coordinated heme-Fe atom of MP11-Fe(III). In fact, cyanide binding to the sixth coordination position of the heme-Fe atom inhibits the MP11-Fe(III)-catalyzed isomerization of peroxynitrite to NO3(-). The values of the first-order rate constant (k0) for isomerization of peroxynitrite to NO3(-) in the presence of the MP11-Fe(III)-CN complex are superimposable to those obtained in the absence of MP-Fe(III). Values of kon for peroxynitrite isomerization to NO3(-) by MP11-Fe(III) overlap those obtained for penta-coordinated cardiolipin-cytochrome c complex and for carboxymethylated cytochrome c in absence and presence of cardiolipin. Present results highlight the role of the heme-Fe(III) co-ordination state in the modulation of cytochrome c reactivity.
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MEDLINE
Assunto principal:
Peroxidases
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Compostos Férricos
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Ácido Peroxinitroso
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article