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Transcriptional response of stress-regulated genes to industrial effluent exposure in the cockle Cerastoderma glaucum.
Karray, Sahar; Tastard, Emmanuelle; Moreau, Brigitte; Delahaut, Laurence; Geffard, Alain; Guillon, Emmanuel; Denis, Françoise; Hamza-Chaffai, Amel; Chénais, Benoît; Marchand, Justine.
Afiliação
  • Karray S; EA 2460 Mer Molécules Santé, Institut Universitaire Mer et Littoral - FR3473 CNRS, Université du Maine - Le Mans, 72085, Le Mans Cedex, France.
  • Tastard E; Laboratoire d'Ecotoxicologie Marine et Environnementale, Université de Sfax, Sfax, Tunisia.
  • Moreau B; EA 2460 Mer Molécules Santé, Institut Universitaire Mer et Littoral - FR3473 CNRS, Université du Maine - Le Mans, 72085, Le Mans Cedex, France.
  • Delahaut L; EA 2460 Mer Molécules Santé, Institut Universitaire Mer et Littoral - FR3473 CNRS, Université du Maine - Le Mans, 72085, Le Mans Cedex, France.
  • Geffard A; UMR-I 02 Stress Environnementaux et Biosurveillance des Milieux aquatiques (SEBIO), Université de Reims Champagne-Ardenne, Moulin de la Housse, BP 1039, 51687, Reims Cedex 2, France.
  • Guillon E; UMR-I 02 Stress Environnementaux et Biosurveillance des Milieux aquatiques (SEBIO), Université de Reims Champagne-Ardenne, Moulin de la Housse, BP 1039, 51687, Reims Cedex 2, France.
  • Denis F; UMR 7312 CNRS-URCA Institut de Chimie Moléculaire de Reims (ICMR) Groupe Chimie de Coordination, Université de Reims Champagne-Ardenne, Moulin de la Housse, BP 1039, 51687, Reims Cedex 2, France.
  • Hamza-Chaffai A; EA 2460 Mer Molécules Santé, Institut Universitaire Mer et Littoral - FR3473 CNRS, Université du Maine - Le Mans, 72085, Le Mans Cedex, France.
  • Chénais B; UMR 7208 CNRS-MNHN-IRD-UPMC, Biologie des Organismes et des Ecosystèmes Aquatiques (BOREA), Concarneau, France.
  • Marchand J; Laboratoire d'Ecotoxicologie Marine et Environnementale, Université de Sfax, Sfax, Tunisia.
Environ Sci Pollut Res Int ; 22(22): 17303-16, 2015 Nov.
Article em En | MEDLINE | ID: mdl-25613800
This study assessed the responses of molecular biomarkers and heavy metal levels in Cerastoderma glaucum exposed for 1 week to two industrial effluents (1%) discharged into the Tunisian coastal area, F1 and F2, produced by different units of production of a phosphate treatment plant. A significant uptake of metals (Cd, Cu, Zn, and Ni) was observed in exposed cockles compared to controls, with an uptake higher for F1 than for F2. A decrease in LT50 (stress on stress test) was also observed after an exposure to the effluent F1. Treatments resulted in different patterns of messenger RNA (mRNA) expression of the different genes tested in this report. Gene transcription monitoring performed on seven genes potentially involved in the tolerance to metal exposure showed that for both exposures, mechanisms are rapidly and synchronically settled down to prevent damage to cellular components, by (1) handling and exporting out metal ions through the up-regulation of ATP-binding cassette xenobiotic transporter (ABCB1) and metallothionein (MT), (2) increasing the mRNA expression of antioxidant enzymes (catalase (CAT), superoxide dismutases, CuZnSOD and MnSOD), (3) protecting and/or repairing proteins through the expression of heat shock protein 70 (HSP70) mRNAs, and (4) increasing ATP production (through the up-regulation of cytochrome c oxidase 1 (CO1)) to provide energy for cells to tolerate stress exposure. The tools developed may be useful both for future control strategies and for the use of the cockle C. glaucum as a sentinel species.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Poluentes Químicos da Água / Cardiidae / Resíduos Industriais Idioma: En Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Poluentes Químicos da Água / Cardiidae / Resíduos Industriais Idioma: En Ano de publicação: 2015 Tipo de documento: Article