Mast cells form antibody-dependent degranulatory synapse for dedicated secretion and defence.
Nat Commun
; 6: 6174, 2015 Jan 28.
Article
em En
| MEDLINE
| ID: mdl-25629393
ABSTRACT
Mast cells are tissue-resident immune cells that play a key role in inflammation and allergy. Here we show that interaction of mast cells with antibody-targeted cells induces the polarized exocytosis of their granules resulting in a sustained exposure of effector enzymes, such as tryptase and chymase, at the cell-cell contact site. This previously unidentified mast cell effector mechanism, which we name the antibody-dependent degranulatory synapse (ADDS), is triggered by both IgE- and IgG-targeted cells. ADDSs take place within an area of cortical actin cytoskeleton clearance in the absence of microtubule organizing centre and Golgi apparatus repositioning towards the stimulating cell. Remarkably, IgG-mediated degranulatory synapses also occur upon contact with opsonized Toxoplasma gondii tachyzoites resulting in tryptase-dependent parasite death. Our results broaden current views of mast cell degranulation by revealing that human mast cells form degranulatory synapses with antibody-targeted cells and pathogens for dedicated secretion and defence.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Degranulação Celular
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Sinapses Imunológicas
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Mastócitos
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Anticorpos Monoclonais
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article