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CXXC5 is a negative-feedback regulator of the Wnt/ß-catenin pathway involved in osteoblast differentiation.
Kim, H-Y; Yoon, J-Y; Yun, J-H; Cho, K-W; Lee, S-H; Rhee, Y-M; Jung, H-S; Lim, H J; Lee, H; Choi, J; Heo, J-N; Lee, W; No, K T; Min, D; Choi, K-Y.
Afiliação
  • Kim HY; 1] Translational Research Center for Protein Function Control, Yonsei University, Seoul, Korea [2] Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.
  • Yoon JY; 1] Translational Research Center for Protein Function Control, Yonsei University, Seoul, Korea [2] Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.
  • Yun JH; Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.
  • Cho KW; Department of Oral Biology, Research Center for Orofacial Hard Tissue Regeneration, College of Dentistry, Yonsei University, Seoul, Korea.
  • Lee SH; 1] Translational Research Center for Protein Function Control, Yonsei University, Seoul, Korea [2] Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.
  • Rhee YM; Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea.
  • Jung HS; Department of Oral Biology, Research Center for Orofacial Hard Tissue Regeneration, College of Dentistry, Yonsei University, Seoul, Korea.
  • Lim HJ; Korea Research Institute of Chemical Technology, Daejeon, Korea.
  • Lee H; Korea Research Institute of Chemical Technology, Daejeon, Korea.
  • Choi J; Bioinformatics and Molecular Design Research Center, Yonsei University, Seoul, Korea.
  • Heo JN; Korea Research Institute of Chemical Technology, Daejeon, Korea.
  • Lee W; Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.
  • No KT; 1] Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea [2] Bioinformatics and Molecular Design Research Center, Yonsei University, Seoul, Korea.
  • Min D; 1] Translational Research Center for Protein Function Control, Yonsei University, Seoul, Korea [2] Department of Molecular Biology, College of Natural Science, Pusan National University, Busan, Korea.
  • Choi KY; 1] Translational Research Center for Protein Function Control, Yonsei University, Seoul, Korea [2] Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.
Cell Death Differ ; 22(6): 912-20, 2015 Jun.
Article em En | MEDLINE | ID: mdl-25633194
ABSTRACT
The positive roles of the Wnt/ß-catenin pathway in osteoblast differentiation and bone mineral density (BMD) maintenance have been clearly demonstrated in both animal experiments and clinical investigations. CXXC finger protein 5 (CXXC5), a recently identified negative regulator of the Wnt/ß-catenin pathway, showed altered cellular localization and function, which were dependent on the cell type in previous studies. However, the in vivo function of CXXC5 has not been clearly investigated yet. Here, we characterized CXXC5 as a negative regulator of osteoblast differentiation and bone formation. Deficiency of CXXC5 resulted in elevated BMD in mice without any severe gross developmental abnormalities. CXXC5 exerted a negative-feedback effect on the Wnt/ß-catenin pathway via Wnt-dependent binding to Dishevelled (Dvl) during osteoblast differentiation. Suppression of the Dvl-CXXC5 interaction using a competitor peptide resulted in the activation of the Wnt/ß-catenin pathway and osteoblast differentiation, and accelerated thickness growth of ex vivo-cultured calvariae. Overall, CXXC5 is a negative-feedback regulator induced by Wnt/ß-catenin signaling that inhibits osteoblast differentiation and bone formation via interaction with Dvl.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Osteoblastos / Beta Catenina / Receptores CXCR5 / Via de Sinalização Wnt Idioma: En Ano de publicação: 2015 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Osteoblastos / Beta Catenina / Receptores CXCR5 / Via de Sinalização Wnt Idioma: En Ano de publicação: 2015 Tipo de documento: Article