Construction, imaging, and analysis of FRET-based tension sensors in living cells.

LaCroix, Andrew S; Rothenberg, Katheryn E; Berginski, Matthew E; Urs, Aarti N; Hoffman, Brenton D

LaCroix, Andrew S; Rothenberg, Katheryn E; Berginski, Matthew E; Urs, Aarti N; Hoffman, Brenton D.

Afiliação

LaCroix AS; Department of Biomedical Engineering, Duke University, Durham, North Carolina, USA.
Rothenberg KE; Department of Biomedical Engineering, Duke University, Durham, North Carolina, USA.
Berginski ME; Department of Biomedical Engineering, Duke University, Durham, North Carolina, USA.
Urs AN; Department of Biomedical Engineering, Duke University, Durham, North Carolina, USA.
Hoffman BD; Department of Biomedical Engineering, Duke University, Durham, North Carolina, USA.

Methods Cell Biol ; 125: 161-86, 2015.

Article em En | MEDLINE | ID: mdl-25640429

Assuntos

Técnicas Biossensoriais; Transferência Ressonante de Energia de Fluorescência/métodos; Imageamento Tridimensional/métodos; Animais; Artefatos; Fenômenos Biomecânicos; Sobrevivência Celular; Fibronectinas/metabolismo; Humanos; Camundongos; Vinculina/metabolismo

Palavras-chave

Biosensor; Forster resonance energy transfer (FRET); Image analysis; Mechanotransduction; Subcellular force measurement; Tension sensor

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Imageamento Tridimensional / Transferência Ressonante de Energia de Fluorescência Idioma: En Ano de publicação: 2015 Tipo de documento: Article

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