Measurement of cell traction forces with ImageJ.
Methods Cell Biol
; 125: 269-87, 2015.
Article
em En
| MEDLINE
| ID: mdl-25640434
ABSTRACT
The quantification of cell traction forces requires three key steps:
cell plating on a deformable substrate, measurement of substrate deformation, and the numerical estimation of the corresponding cell traction forces. The computing steps to measure gel deformation and estimate the force field have somehow limited the adoption of this method in cell biology labs. Here we propose a set of ImageJ plug-ins so that every lab equipped with a fluorescent microscope can measure cell traction forces.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Processamento de Imagem Assistida por Computador
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Software
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Movimento Celular
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Microscopia de Fluorescência
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article