Ligand-directed profiling of organelles with internalizing phage libraries.
Curr Protoc Protein Sci
; 79: 30.4.1-30.4.30, 2015 Feb 02.
Article
em En
| MEDLINE
| ID: mdl-25640897
ABSTRACT
Phage display is a resourceful tool to, in an unbiased manner, discover and characterize functional protein-protein interactions, create vaccines, and engineer peptides, antibodies, and other proteins as targeted diagnostic and/or therapeutic agents. Recently, our group has developed a new class of internalizing phage (iPhage) for ligand-directed targeting of organelles and to identify molecular pathways within live cells. This unique technology is suitable for applications ranging from fundamental cell biology to drug development. This unit describes the methods for generating and screening the iPhage display system, and explains how to select and validate candidate internalizing homing peptide.
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Base de dados:
MEDLINE
Assunto principal:
Organelas
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Técnicas Citológicas
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Técnicas de Visualização da Superfície Celular
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article