Chemical and UV Mutagenesis.
Methods Mol Biol
; 1373: 111-5, 2016.
Article
em En
| MEDLINE
| ID: mdl-25646611
ABSTRACT
The ability to create mutations is an important step towards understanding bacterial physiology and virulence. While targeted approaches are invaluable, the ability to produce genome-wide random mutations can lead to crucial discoveries. Transposon mutagenesis is a useful approach, but many interesting mutations can be missed by these insertions that interrupt coding and noncoding sequences due to the integration of an entire transposon. Chemical mutagenesis and UV-based random mutagenesis are alternate approaches to isolate mutations of interest with the potential of only single nucleotide changes. Once a standard method, difficulty in identifying mutation sites had decreased the popularity of this technique. However, thanks to the recent emergence of economical whole-genome sequencing, this approach to making mutations can once again become a viable option. Therefore, this chapter provides an overview protocol for random mutagenesis using UV light or DNA-damaging chemicals.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Elementos de DNA Transponíveis
/
Biologia Molecular
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Mutação
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article